Fig. 7: RPOC-controlled site-specific state transition of photochromic molecules visualized by stimulated Raman scattering (SRS) microscopy.

A Molecular structures and photoconversion of cis − 1,2-dicyano1,2-bis(2,4,5-trimethyl-3-thienyl)ethene (CMTE). B SRS images at different Raman transitions, active pixels (APXs), and the SRS intensity changes for cells treated with CMTE. The bottom rows show enlarged areas of the selected regions in the top row. CMTE is treated using only the 532 nm laser at the selected APXs. Scale bars: 10 µm for top panels, 5 µm for bottom panels. C SRS intensity of the CMTE signals before and after green light treatment, and the APX intensity along the selected line in panel (B). D SRS signals at 1510 cm⁻¹ for CMTE (red), endoplasmic reticulum (ER) signals from ER-Tracker (green), and APX for the 532 nm laser treatment (magenta). The ER boundary is outlined in green curves. The SRS intensity change at 1510 cm⁻¹ shows where the CMTEs are converted to the open form in the cell. Adapted with permission from ref. ⁴⁶ Scale bars: 5 µm.