Table 1 Chemicals used for tissue section preparation
| Â | Name | Manufacturer | Item number |
|---|---|---|---|
1 | Xylene | Fisher Scientific | X3P-1GAL |
2 | Ethanol (100%) | Fisher Scientific | BP28184 |
3 | 10X Antigen retrieval buffer (PH6) | Invitrogen | 00-4955-58 |
4 | Sodium borohydride | Sigma-Aldrich | 213462-25 G |
5 | Triton X-100 | Â | Â |
6 | Hydrogen peroxide 30% W/W | Sigma-Aldrich | 216763-100 ml |
7 | Sodium hydroxide, Pellet AR (ACS) | Mallinckrodt Chemicals | 7708-10 |
8 | 10X PBS with calcium and magnesium | Fisher Scientific | BP399 |
9 | L-Glycine | Sigma-Aldrich | 50046-50 G |
10 | Urea | Sigma-Aldrich | U5378-500G |
11 | Guanidium chloride (GC) | Millipore-Sigma | 369075-1KG |
12 | N-acetyl-cysteine (NAC) | Sigma-Aldrich | A7250-100G |
13 | Ammonium chloride(NH4Cl) | Â | Â |
14 | Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) | Sigma-Aldrich | C4706-10G |
15 | Maleimide | Sigma-Aldrich | 129585-2 G |
16 | 12 M Hydrochloric acid | Fisher Scientific | A144-500 |
17 | Trizma base | Sigma-Aldrich | T1503-25KG |
18 | Bovine serum albumin | Sigma-Aldrich | A9647-100G |
