Fig. 1: Example of additional information provided by fluorescence polarization microscopy (FPM). | npj Imaging

Fig. 1: Example of additional information provided by fluorescence polarization microscopy (FPM).

From: High contrast fluorescence polarization microscopy through double tagged photoswitchable fluorescent proteins

Fig. 1

a Setup for fluorescence polarization microscopy by rotation of the excitation polarization35. b Conventional image of A549 lung cancer cells labeled by LAURDAN. c Image of only the modulation amplitude, A (c.f Eq. (1)), observed in FPM. d Image of the amplitude, A, of only one selected frame excited with a distinct polarization angle, α. e Modulation amplitude image of c, color-coded by the observed modulation phases (x, cf. Equation (2)) of different membrane parts.

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