Fig. 3: Live cell imaging and mIF panels of immune subpopulations in primary resected tumours and corresponding tumoroids. | npj Imaging

Fig. 3: Live cell imaging and mIF panels of immune subpopulations in primary resected tumours and corresponding tumoroids.

From: Kidney tumoroid characterisation by spatial mass spectrometry with same-section multiplex immunofluorescence uncovers tumour microenvironment lipid signatures associated with aggressive tumour phenotypes

Fig. 3

A Live cell imaging of single cell suspension and tumoroids showing apoptotic regions (annexin V, red) and immune cell presence (CD45, green). B mIF of FFPE primary tumour and tumoroids confirming immune (CD45, green) and mesenchymal, possible endothelial (vimentin, yellow) cell populations. C FFPE sections of primary tumours and matched tumoroids at Day 21 (n = 3) stained for six immune cell markers (CD68, CD56, CD11b, CD20, CD3, and ICOS) alongside the epithelial cell marker cytokeratin (CK).

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