Fig. 6: Characterisation of primary resected RCC tissue and tumoroids (case 622) using DESI-MSI, histochemical staining and mIF. | npj Imaging

Fig. 6: Characterisation of primary resected RCC tissue and tumoroids (case 622) using DESI-MSI, histochemical staining and mIF.

From: Kidney tumoroid characterisation by spatial mass spectrometry with same-section multiplex immunofluorescence uncovers tumour microenvironment lipid signatures associated with aggressive tumour phenotypes

Fig. 6

A H&E, ORO and PAS staining of primary tissue. A1, B1 Magnified regions of primary tissue of cancer cells presenting with dedifferentiated (green and purple) or high extracellular matrix-enriched (pink and yellow) patterns. B Primary tissue mIF images of PanCK (pink) and a composite of NucBlue™ (blue), Ki67 (green), CD105 (red) and PanCK (pink). C pLSA of lipids and metabolites. D and F DESI-MSI ion images of m/z 707.5056 [M–H]− and m/z 716.5233 [M-H]− of primary tissue and tumoroids, respectively, with highlighted regions corresponding to H&E and mIF magnifications (A1, B1 and E). E H&E and mIF images of tumoroids showing cancer cells with variable PanCK expression.

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