Fig. 6: CCA1 circadian mutants do not display SA induced resistance to Pseudomonas.

Col-0, CCA1ox, cca1lhy, TOC1ox and toc1-101 plants were grown under 12/12 light/dark conditions for 3 weeks. At dawn on day 21 (LL0), the plants were released into constant light conditions. At LL24 the plants were sprayed with 1 mM SA or mock (water). At LL48 the plants were infected with Pseudomonas syringae pv. maculicola (Psm). The level of bacterial growth was measured 3 days post infection. a Leaves representative of the visual median level of infection at 3 days post-infection for each genotype and treatment. b The infection level at 3 days post-infection expressed by colony forming units (CFU, y-axis is logarithmic) in leaf disk extracts. Error bars represent mean ± SEM (n = 8 individual leaves). A two-way ANOVA and Tukey’s HSD Test were preformed to analyse the differences in bacterial growth between treatments and genotypes. The letters indicate significant differences between groups (p < 0.05). The data shown here are from a single experiment, representative of 3 independent experiments (Col-0 versus CCA1ox, cca1lhy or TOC1ox [Supplementary Fig. 5a–c]) or 2 independent experiments (Col-0 versus toc1-101 [Supplementary Fig. 5c, d]).