Fig. 1: Schema of the brain region sampled, the collection time/condition, and a plot of the number of APA sites per gene.
From: Sleep and diurnal alternative polyadenylation sites associated with human APA-linked brain disorders
A The region of the central rat forebrain that was collected and used for RNA extraction is bounded by dotted lines and labeled ‘forebrain’ (brain illustration was generated by modifying an image obtained in Motifolio (Motifolio Inc., Ellicott City, MD, USA). B For sleep homeostasis experiments, rats were sleep-deprived for 6 h and allowed to recover for 0–8 h before tissue extraction. Three of the time-matched controls (no SD) were shared with the diurnal experiment and one additional time point (no SD at ZT8), was not in common. For the diurnal analysis, samples were taken at 4 h intervals from ZT2 until ZT22. Five biological replicates were used for all data points. C A diagram of a generic gene shows different types of APAs: within an internal exon (1); within an early intron (2); following an internal exon (3); within the longest documented 3′ UTR (4); at the terminus of the longest documented 3′ UTR (5); and distal to longest documented 3′ UTR. D WTTS-seq PAS results; the number of genes on the x-axis (log10 scale) are plotted against the number of APA sites per gene.
