Fig. 2: Deletion of Arntl in adipocytes reduced lipolysis. | npj Biological Timing and Sleep

Fig. 2: Deletion of Arntl in adipocytes reduced lipolysis.

From: Deletion of Arntl, a component of the molecular clock, in adipocytes leads to cellular hypertrophy by increasing insulin sensitivity via FGF21

Fig. 2

a Left, representative western blot of total hormone-sensitive lipase (HSL), phosphorylated HSL, total cAMP response element binding protein (CREB), and phosphorylated CREB in the eWAT. β-ACTIN was used as loading control. Right, the relative intensities of the bands were analyzed using Amersham ImageQuant TL (n = 3). b cAMP levels (n = 7). c PKA activity (n = 4). d Phosphodiesterase (PDE) activity (n = 4). Data are represented as the means ± SD and were compared using two-way ANOVA with Bonferroni’s post-hoc test (a) or Tukey’s post-hoc test (b–d). *P < 0.05, **P < 0.01, ***P < 0.001, relative to Arntlflox/flox mice at the same timepoint and #P < 0.05, or ##P < 0.01 relative to ZT10 in the same genotype.

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