Fig. 3: Deletion of Arntl in adipocytes increased insulin sensitivity in the eWAT. | npj Biological Timing and Sleep

Fig. 3: Deletion of Arntl in adipocytes increased insulin sensitivity in the eWAT.

From: Deletion of Arntl, a component of the molecular clock, in adipocytes leads to cellular hypertrophy by increasing insulin sensitivity via FGF21

Fig. 3

a Serum insulin levels in mice (n = 3–5). b Blood glucose levels during the insulin tolerance test (ITT) at ZT10 and ZT22 and the area over the curve (AOC) was calculated for respective group (right, n = 4–5). c,d [3H]-2-deoxyglucose (2-DG) uptake rate in tissue at ZT10 (left) and ZT22 (right). c eWAT. d Skeletal muscle. e,f Top, representative western blot of total AKT and phosphorylated AKT at ZT10 and ZT22. β-ACTIN was used as a loading control. Bottom, the relative intensities of the bands were analyzed using Amersham ImageQuant TL (n = 3). e eWAT. f Skeletal muscle. Data are presented as the mean ± SD and were compared using two-way ANOVA with Bonferroni’s post-hoc test (a,b left and center) or Tukey’s post-hoc test (b right, c–f). *P < 0.05, **P < 0.01, ***P < 0.001 relative to Arntlflox/flox mice at the same timepoint or treatment and ##P < 0.01, or ###P < 0.001 relative to ZT10 in the same genotype.

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