Fig. 2: SD by novel object prolongs wakefulness and decreases the duration of NREM and REM sleep but the sleep is recovered over the 24 h on the seventh day of SD. | npj Biological Timing and Sleep

Fig. 2: SD by novel object prolongs wakefulness and decreases the duration of NREM and REM sleep but the sleep is recovered over the 24 h on the seventh day of SD.

From: Introduction of novel objects to the home cage of mice repeatedly disturbs sleep for seven days with minimal stress induction

Fig. 2

Mice (n = 6–7) had EEG/EMG/video recording on the baseline, first day of SD, and seventh day of SD to validate the SD by novel objects. Duration of wakefulness on all days at A ZT2-6 (SD) B ZT6-2 (20-hour recovery sleep) and C Total 24 h. D Duration of wakefulness per hour. Duration of NREM sleep on all days at E ZT2-6 (SD) F ZT6-2 (20-h recovery sleep) and G Total 24 h. H Duration of NREM sleep per hour. Duration of REM sleep on all days at I ZT2-6 (SD) J ZT6-2 (20-h recovery sleep) and K Total 24 h. L Duration of REM sleep per hour. For the bar plots (A–C, E–G, I–K), data from the same mouse is connected by a line on bar graphs. Repeated measures and paired mixed-effect analysis with the Geisser-Greenhouse correction and Tukey’s multiple comparisons tests, was used to analyze the data illustrated in the bar plots, *p < 0.05, **p < 0.01, ***p < 0.001. For figures (D, H, L), the value plotted at ZT2 is the sum of [2,3[and so on. The shaded gray indicates the dark phase (ZT12-0) and the colored shading indicates the timing of SD (ZT2-6). The line-types indicate the mean across animals and the colored shading around the line indicates SEM. *p < 0.5 indicates significance for baseline vs 1st day of SD and ¤p < 0.05 indicates significance for baseline vs 7th day of SD.

Back to article page