Fig. 3: The relative EEG power of NREM sleep increases at ZT4 on the first day of SD and at ZT3 and ZT4 on the seventh day of SD. | npj Biological Timing and Sleep

Fig. 3: The relative EEG power of NREM sleep increases at ZT4 on the first day of SD and at ZT3 and ZT4 on the seventh day of SD.

From: Introduction of novel objects to the home cage of mice repeatedly disturbs sleep for seven days with minimal stress induction

Fig. 3

The relative EEG power was calculated for wakefulness, NREM sleep, and REM sleep from the mice (n = 6–7) during the 24 h recordings. Power density plot for A NREM sleep, B REM sleep, and C wakefulness during 24 h on all days. D Relative delta EEG power for NREM sleep per hour for all days. The shaded gray indicates the dark phase (ZT12-0) and the colored shading indicates the timing of SD (ZT2-6). Not all animals entered NREM sleep every hour; thus, the line-types indicate the mean across animals and the colored shading around the line indicates SEM. A repeated measures and paired mixed-effect model with the Geisser-Greenhouse correction with a Tukey’s multiple comparisons test was performed to compare relative EEG power per hour for NREM sleep. Baseline vs 1st day of SD - *p < 0.05 and baseline vs 7th day of SD ¤p < 0.05.

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