Fig. 1: Selection increases resistance to mites and RNA-seq studies reveal altered metabolism-associated factors following selection for ectoparasite resistance.

A Artificial selection for mite resistance resulted in significantly reduced susceptibility to mites in selected (S) versus control (C) lines after 21 generations. * denotes significance at P < 0.05 between control and selected lines (F = 16.24, d.f. = 2,5, P = 0.001). Error bars represent the 95% confidence intervals. B Gene ontology categories enriched in differentially expressed genes between S and C from a full analysis of all samples (nested within the replicate, revealing factors generally associated with metabolism, FDR, P < 0.05). There were three biological replicates of RNA-Seq data for each treatment (S and C) of the three artificial selection replicates.