Fig. 1: Comparative phase-shift responses of adipose tissue and fibroblasts to bright light exposure and corticosterone.
From: Light does not phase shift the circadian clock of subcutaneous adipose tissue in vitro
A Incubation set-up of the cell cultures on top of the white light box. B The relative spectral power distribution of the white light box was constantly set to a correlated color temperature of 5000 K. C Detrended bioluminescence trace of adipose tissue explant from PER2::LUC mice treated with either vehicle (DMSO), 100 nM corticosterone (CORT) or 4–h incubation on a white light box (LIGHT), measured in relative luminescence units (RLU), n ≥ 4 biological replicates (dishes of tissue from 5 mice), mean ± SEM. UT= untreated. D Data from A, used during quantitative phase analysis in (G). Detrended bioluminescence trace of PER2::LUC mouse fibroblasts treated with either DMSO, CORT, or LIGHT measured in RLU, n = 4 biological replicates (dishes of fibroblasts), mean ± SEM. F Data from C, used during quantitative phase analysis in (E). G Quantification of treatment-induced change (Δ) in phase (h) from (B) and (D), n = 4, mean ± SEM, one-way ANOVA (OWA), Holm–Sidak’s MCT, ****= p < 0.0001, *** = p = 0.001, ns = p > 0.05. H, I Circular plots depicting quantification of treatment-induced change in phase (h) from (D) and (F), respectively. black = untreated, blue = DMSO, red = CORT, purple = LIGHT. Hours are plotted around the circle axis, the mean of each condition is depicted by colored lines, and SEM is the length of the colored bar around the circle.
