Fig. 2: Basal comparison of regulatory phosphosites across 12 AMPK complexes. | npj Metabolic Health and Disease

Fig. 2: Basal comparison of regulatory phosphosites across 12 AMPK complexes.

From: AMPK phosphosite profiling by label-free mass spectrometry reveals a multitude of mTORC1-regulated substrates

Fig. 2

A AMPK complexes were FLAG-immunoprecipitated from HEK293T/17 cells incubated in complete growth media and immunoblotted as indicated. Representative immunoblots from 3 independent experiments are shown. Quantitation of (B) α-pT172, C β1-pS108, and (D) α-pS487. Data presented as mean phosphorylation (a.u. arbitrary units) ± SEM, n = 3. Statistical analyses were performed by one-way ANOVA with Dunnett’s multiple-comparisons test. **P < 0.01, ****P < 0.0001 vs. respective γ1 complex. n.s. not significant; n.d. not determined.

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