Table 4 Primers used for cloning

_6xHisα2(α1ST) & _6xHisα2(α1RIM)

(pET DUET-1 MCS1)

Forward

Reverse

5′ GGTAGGATCCGCTGAGAAGCAGAAGCACGACG 3′

5′ GCATGCGGCCGCTCAACGGGCTAA

AGTAGTAATCAGACTGG 3′

α2_{no tag} (α1ST)

(pcDNA3.1(−))

Forward

Reverse

5′ GGTACTCGAGATGGCTGAGAAGCAGAAGC 3′

5′ GCATAAGCTTTCAACGGGCTAAAGTAG 3′

γ1_FLAG

(pcDNA3.1(−))

Forward

Reverse

5′ GCATGCGGCCGCATGGAGACGGTCATTTCTTCA 3′

5′GCATGAATTCTCACTTGTCGTCATCGTCTTT

GTAGTCTCCACCGGGCTTCTTCTCTCCACC 3′

γ2_FLAG

(pcDNA3.1(−))

Forward

Reverse

5′ GCATGCGGCCGCATGGGAAGCGCGGTTAT 3′

5′ GCATGAATTCTCACTTGTCGTCATCGTCTTT

GTAGTCTCCACCCTCCGTTTCTGTCTCCT 3′

Lambda phosphatase

Forward

Reverse

5′ GCATGGATCCCGGTACTACGAAAAAATCGACG 3′

5′ GCATGCGGCCGCTTAGGCACCTTCGCCCTGAACC 3′