Fig. 2: Effects of MFG-E8 deficiency in the STAM-MASH model.

a Total NAS scores (n = 5–6). b Steatosis scores (n = 5–6). c Inflammation scores (n = 5–6). d Ballooning scores (n = 5–6). e RT–qPCR analysis of Tnf mRNA abundance in liver (n = 4–5). f RT–qPCR analysis of Ccl2 mRNA abundance in liver (n = 4–5). g RT–qPCR analysis of Il6 mRNA abundance in liver (n = 4–5). h, RT–qPCR analysis of Adgre1 mRNA abundance in liver (n = 4–5). i RT–qPCR analysis of Col1a1 mRNA abundance in liver (n = 4–5). j Serum aspartate aminotransferase (AST) levels (n = 4–5). k Serum alanine aminotransferase (ALT) levels (n = 4–5). l Representative F4/80 immunohistochemical staining in liver of WT mice (+/+). m Representative F4/80 staining in liver of MFG-E8 KO mice (–/–). n Quantification of F4/80-positive cells per high-power field (0.2 mm²) (n = 5–9). o Quantification of hepatic crown-like structures (hCLS) per high-power field (0.2 mm²) (n = 5–9). p Representative Sirius Red–stained liver section from WT mice. q Representative Sirius Red–stained liver section from MFG-E8 KO mice. r Quantification of relative Sirius Red–positive area (n = 4–5). s Kaplan–Meier survival curves in the STAM-MASH model (n = 21–22). Line colors in the panel indicate the genotype: red, WT (+/+); purple, MFG-E8 KO (–/–). t Survival duration in the STAM-MASH model (n = 21–22). Throughout Fig. 2, markers indicate the genotype of STAM-MASH mice: red circles, WT (+/+); green squares, heterozygous MFG-E8 knockout (+/−); purple triangles, homozygous knockout (−/−). Panels (l–o) show 5–6-week-old mice, which are younger than the 8-week-old animals used in most other experiments. The P-value in panel s was determined using the log-rank test. The amounts of mRNAs were normalized to 18S rRNA. All quantitative data, except those in (s), represent means ± s.e.m. *P < 0.05; **P < 0.01 (Student’s t-test or one-way ANOVA with Tukey’s post-hoc test).