Table 1 Summary of interaction parameters between PR65 and SMAP ATUX-8385 found from single molecule NOT measurements (n = 8)
From: Direct observation of small molecule activator binding to single PR65 protein
τoff(s) | τon(s) | koff (s−1) | kon (s−1 μM−1) | ΔG∘ (kcal/mol) | KD (μM) |
|---|---|---|---|---|---|
0.40 ± 0.06 | 0.37 ± 0.03 | 2.52 ± 0.42 | 0.19 ± 0.02 | −6.72 ± 2.5 | 13.6 ± 2.5 |
