Fig. 4: sdRT-QuIC analysis of CSF.
From: A same day α-synuclein RT-QuIC seed amplification assay for synucleinopathy biospecimens
Representative analyses of single PD and NS control CSF specimens using a RT-QuICR and b sdRT-QuIC. Traces show means ± SD of ThT fluorescence from quadruplicate reactions. c 1/TTT and d ThT maxima within 20 h of sdRT-QuIC reactions seeded with PD (n = 4) and NS (n = 4) CSFs. The same CSF samples were used in the respective RT-QuICR and sdRT-QuIC assays. Data points represent means of quadruplicate reactions from individual cases, and bars represent the mean ± SD of the means from all cases. e End-point dilutions of a PD CSF by sdRT-QuIC. Each trace represents the mean ± SD ThT fluorescence of quadruplicate wells. f log SD50/15 µl values for PD CSFs (n = 4). Statistical significance is denoted as *p < 0.05, **p < 0.01, ***p < 0.001.
