Fig. 1: Free energy and phase diagrams of protein aggregation.
From: Mechanism of amyloid fibril formation triggered by breakdown of supersaturation
a Free energy diagram with (red) and without (black) physical stress. The aggregation reaction starts from a monomeric state to amyloid fibrils or amorphous aggregates. ΔG≠ is transition free energy. b Concentration-dependent solute and precipitation phase diagram common to native crystals and aggregates of denatured proteins. The phase boundaries between amorphous and labile regions or between labile and metastable regions under quiescence are altered by agitation and ultrasonication70.
