Fig. 5: Application of HANABI-2000 for DRA.
From: Mechanism of amyloid fibril formation triggered by breakdown of supersaturation
a Illustration of HANABI-2000 experiment using sera from dialysis patients (DT(+, Pre), DT(+, Post)) and non-dialysis controls (DT(−)). Sera were collected from identical dialysis patients before (DT(+, Pre)) and after (DT(+, Post)) a single maintenance dialysis. b Representative kinetics with sera collected from non-dialysis controls (DT(−)) and dialysis patients (DT(+, Pre)). Control (Serum(−)) does not contain sera and forms amyloid fibrils without inhibitory effects of sera. c The effects of dialysis-treated and non-dialysis-treated sera on β2m amyloid formation were compared in terms of the lag time (left) and ThT fluorescence (right). P-values were calculated by the unpaired one-sided t-test. d β2m concentrations in non-dialysis control group (DT(−)) and dialysis patient group (DT(+, Pre)). In the non-dialysis group, glomerular filtration rate (GFR) is used as an index of kidney function. e Amyloid formation of β2m with 5% (v/v) sera from identical dialysis patients before (DT(+, Pre)) and after (DT(+, Post)) a single maintenance dialysis. f The effects of sera with and without maintenance dialysis on amyloid formation. g Effects of maintenance dialysis on serum β2m concentration and body weight of dialysis patients before (DT(+, Pre)) and after (DT(+, Post)) maintenance dialysis. Relative changes in the two parameters are shown compared to their values before maintenance dialysis. The figures were modified under the terms of the Creative Commons Attribution CC BY license from Nakajima et al.49.
