Table 1 Methods to accelerate amyloid formation.
From: Mechanism of amyloid fibril formation triggered by breakdown of supersaturation
Methods | Comments | References |
|---|---|---|
PMCA | PMCA amplifies seeds in the presence of monomers by breakage and elongation of fragmented amyloid fibrils under ultrasonication. | |
RT-QuIC | Similar to PMCA, RT-QuIC amplifies seeds in the presence of monomers by agitation-dependent breakage and elongation of fragmented amyloid fibrils. | |
IP/RT-QuIC | Seeds are immunoprecipitated and amplified by RT-QuIC. | |
Micro-QuIC | Acoustofluidic mixing allows for homogeneous mixing of reagents and seeds are efficiently amplified. | |
HANABI | HANABI focuses on spontaneous amyloid formation by breaking supersaturation and is also effective in amplifying seeds using the same mechanism as PMCA. | |
Flow/extended flow | Flow-induced shear stress in microfluidic channels promotes amyloid formation. | |
Beads or nanoparticles | Beads and particles promote amyloid formation through agitation-dependent breakage and interaction with the bead surface. |
