Fig. 5: Implementation of signal recovery protocol on E-AB sensors against other targets. | npj Biosensing

Fig. 5: Implementation of signal recovery protocol on E-AB sensors against other targets.

From: General approach to achieving electrochemical aptamer-based sensor sensitivity of buffer in blood plasma

Fig. 5

We show data for two additional E-AB sensors using off-the-shelf aptamers against tobramycin (a, b) hydroxyquinoline. In both cases the sensors EC50 appeared better in buffered saline relative to plasma, with EC50 values of 190 µM vs. 996 µM for tobramycin, respectively, and EC50 values of 900 µM vs. 4.5 mM for hydroxyquinoline. The hill equation is fit to n = 8 measurements for each concentration point, with error bars denoting the standard deviation. The implementation of the protein precipitation and filtration protocol achieves either equal or an improvement of EC50 in both cases. c The filtrate curve for tobramycin shows an EC50 of 281 µM. d The filtrate curve for hydroxyquinoline shows an EC50 of 1.08 µM. The hill equation is fit to n = 15 measurements for each concentration point, with error bars denoting the standard deviation for both c, d.

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