Fig. 2: Plasmonically enhanced DNA-RNA hybridized assay.
From: Plasmon-enhanced bioassay for amplification-free detection and quantification of SARS-CoV-2 RNA
A Vis–NIR extinction spectra of plasmonic-fluor showing a longitudinal plasmonic extinction band at 650 nm (inset shows the TEM image of plasmonic-fluor-650). B Fluorescence intensity of conventional fluor and plasmonic-fluor bound to biotinylated BSA immobilized at the bottom of a microtiter well, showing nearly 1100-fold brighter fluorescence intensity of plasmonic-fluor compared to conventional fluor. Error bars, s.d. (n = 2 independent tests). Data statistically significant **P < 0.01 by two-tailed unpaired t-test. C Histogram and D fluorescence intensity map corresponding to hybridized RNA-DNA, hybridized RNA-DNA-biotin, hybridized RNA-DNA-biotin-Det-biotin, single-strand RNA, single-strand DNA-biotin, single-strand DNA, non-specific RNA solution extracted from 3T3 cell line, non-specific RNA solution extracted from 3T3 cell line incubated with DNA-biotin (10 fg/ml), non-specific RNA solution extracted from 3T3 cell line incubated with DNA probe (10 fg/ml), and Blank (reaction buffer). Error bars, s.d. (n = 2 independent experiments). Data statistically significant. ****P < 0.0001, ***P < 0.001 by One way-ANOVA.
