Fig. 3: LOD comparison of RNA/DNA-biotin-ELISA, Method I, Method II, and Method III.
From: Plasmon-enhanced bioassay for amplification-free detection and quantification of SARS-CoV-2 RNA
A Plots showing N gene dose-dependent standard curve of RNA/DNA-biotin ELISA. The LOD and LLOQ were found to be 1.73 fM and 10.8 fM, respectively; B Plots showing N gene dose-dependent standard curve of Method I (RNA/DNA-Detection antibody-biotin-PFLISA). The LOD and LLOQ were found to be 7 aM and 53 aM, respectively. C Plots showing N gene dose-dependent standard curve of Method II (RNA/DNA-biotin-PFLISA assay). The LOD and LLOQ were found to be 37.4 aM and 139 aM, respectively; D Plots showing N gene dose-dependent standard curve of Method III (RNA/DNA-biotin-Detection antibody-biotin-PFLISA). The LOD and LLOQ were found to be 5.34 aM and 40.5 aM, respectively. For all assays, n = 2 technical replicates, and data is presented as mean ± sd. Data points corresponding to blanks are included as the lowest concentration in the plots.
