Fig. 5: DNA-biotin-PFLISA assay and qRT-PCR of patients’ samples.
From: Plasmon-enhanced bioassay for amplification-free detection and quantification of SARS-CoV-2 RNA
A The viral RNA Ct of delta-SARS-CoV-2 patients’ samples, alpha/beta-SARS-CoV-2 patients’ samples, SARS-CoV-2 negative individuals, and other viruses (Corona-NL63 positive, Rhinovirus positive, and Adenovirus positive) infected patients’ samples. The COVID-positive threshold was set at 39. The Ct value of undetectable samples was set at 45. B The viral RNA concentration quantified by DNA-biotin-PFLISA assay of delta-SARS-CoV-2 patients’ samples, alpha/beta-SARS-CoV-2 patients’ samples, SARS-CoV-2 negative individuals and other viruses (Corona-NL63 positive, Rhinovirus positive, and Adenovirus positive) infected patients’ samples. The positive threshold was set at LOD (13 aM) of the assay. All the samples that exhibited a lower fluorescence signal than the LOD of the assay are indicated as Not Detected (ND). C The correlation between the ORF1ab gene (Pearson’s r2 = 0.37) was tested by DNA-biotin-PFLISA assay and qRT-PCR. D Comparison of p-FLISA and ELISA in detecting SARS-CoV-2 RNA in a subset of patient samples. The dashed line represented the LOD of each of the assays.
