Abstract
Study design:
A case–control evaluating seminal citrate in patients with spinal cord injury (SCI).
Objective:
Several studies have shown neurological prostatic dysfunction in patients with SCI, as confirmed by low levels of seminal prostate-specific antigen (PSA), which is used as a parameter of gland activity. However, seminal citrate, produced almost exclusively by the prostate, could also be used as a marker of prostate function. Thus, the objective of this study was to determine whether SCI causes any changes in seminal citrate concentration and to compare the results obtained for patients and healthy men (controls).
Setting:
The study was carried out in Brazil.
Methods:
We studied 30 men with SCI aged on average 37.77±10.04 years and 30 controls aged on average 38.03±10.06 years. Blood and semen samples were collected after 3 days of abstinence from ejaculation. Fifteen minutes after collection, semen was stored in liquid nitrogen and the samples were submitted to 1H nuclear magnetic resonance (1H NMR). Serum was stored at a controlled temperature of −70 to −79 °C and later used for the determination of testosterone, prolactin and total PSA using an AxSYM instrument and Abbott reagents.
Results:
The median concentration of seminal citrate was significantly lower in patients than in controls (521.65 versus 858.30 mg per 100 ml, P<0.001).
Conclusions:
Patients with SCI have a significant reduction of seminal citrate as a consequence of neurological dysfunction of the prostate.
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References
Lynne CM, Aballa TC, Wang TJ, Rittenhouse HG, Ferrell SM, Brackett NL . Serum and semen prostate specific antigen concentrations are different in young spinal cord injured men compared to normal controls. J Urol 1999; 162: 89–91.
Green JT, Aballa TC, Lynne CM, Brackett NL . Seminal fructose is decreased in ejaculates from men with spinal cord injury. Fertil Steril 1998; 70 (Suppl 1): 200s.
Alexandrino AP, Rodrigues MAF, Matsuo T . Evaluation of serum and seminal levels of prostate specific antigen in men with spinal cord injury. J Urol 2004; 171: 2230–2232.
Iwakiri J, Grandbois K, Wehner N, Graves HC, Stamey T . An analysis of urinary prostate specific antigen before and after radical prostatectomy: evidence for secretion of prostate specific antigen by the periurethral glands. J Urol 1993; 149: 783–786.
Shortliffe LMD, Sellers RG, Schachter J . The characterization of nonbacterial prostatites: search for an etiology. J Urol 1992; 148: 1461–1466.
Mann T . Fructose, polyols, and organic acids. In: Mann T (ed). The Biochemistry of Semen and of the Male Reproductive Tract. Methuen: London, 1964, pp 237–264.
Tomlins AM, Foxall PJD, Lynch MJ, Parkinson J, Everett JR, Nicholson JK . High resolution H NMR spectroscopic studies on dynamic biochemical processes in incubated human seminal fluid samples. Biochim Biophys Acta 1998; 1379: 367–380.
Maynard Jr FM, Bracken MB, Creasey G, Ditunno Jr JF, Donovan WH, Ducker TB et al. International standard for neurological and functional classification of spinal cord injury. Spinal Cord 1997; 35: 266–274.
Daniel WW . Biostatistics: a Foundation for Analysis in the Health Sciences, 2nd edn. John Wiley: New York, NY, 1995.
Dunn OJ . Multiple comparisons using rank sums. Technometrics 1964; 6: 241–252.
Huang HF, Li MT, Linsenmeyer TA, OttenWeller JE, Pogach L M, Irwin RJ . The effects of spinal cord injury on status of messenger ribonucleic acid for TRPM 2 and androgen receptor in the prostate of rat. J Androl 1997; 18: 250–256.
Mcvary RT, Razzaq A, Lee C, Venegas MF, Rademaker A, Mckenna KE . Growth of the rat prostate gland is facilitated by the autonomic nervous system. Biol Reprod 1994; 51: 99–104.
Frisbie JH, Kumar S, Aguilera EJ, Yalla S . prostate atrophy and spinal cord lesions. Spinal Cord 2006; 44: 24–27.
Kline EE, Treat EG, Averna TA, Davis MS, Smith AY, Sillerud LO . Citrate concentrations in human seminal fluid and expressed prostatic fluid determined via 1H nuclear magnetic resonance spectroscopy outperform prostate specific antigen in prostate cancer detection. J Urol 2006; 176: 2274–2279.
Dondero F, Sciarra F, Isidori A . Evaluation of relationship between plasma testosterone and human seminal citric acid. Fertil Steril 1972; 23: 168–171.
Kurhanewicz J, Vigneron DB, Nelson SJ, Hricak H, Macdonald JM, Konety B et al. Citrate as an in vivo marker to discriminate prostate cancer from benign prostatic hyperplasia and normal prostate peripheral zone: detection via localized proton spectroscopy. Urology 1995; 45: 459–466.
Abrahamsson PA, Wadstrrom LB, Alumets J, Falkmer S, Grimelius L . Peptide-hormone and serotonin-immunoreactive cells in normal and hyperplastic prostate glands. Pathol Res Pract 1986; 181: 675–683.
Costello LC, Franklin RB . Concepts of citrate production and secretion by prostate: 2 hormonal relationships in normal and neoplastic prostate. Prostate 1991; 19: 181–205.
Claus-walker J, Scurry M, Carter RE, Campos RJ . Steady state hormonal secretion in traumatic quadriplegia. J Clin Endocrinol Metab 1977; 44: 530–535.
Costello LC, Franklin RB . The clinical relevance of the metabolism of prostate cancer; zinc and tumor suppression: connecting the dots. Mol Cancer, [serial online] 2006 May [cited 5 November 2006] 5: 5–17. Available from: URL: http://www.molecular-cancer.com/content/pdf:1476-4598-5-17.pdf.
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Alexandrino, A., Rodrigues, M., Matsuo, T. et al. Evaluation of seminal citrate level by 1H nuclear magnetic resonance spectroscopy in men with spinal cord injury. Spinal Cord 47, 878–881 (2009). https://doi.org/10.1038/sc.2009.62
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DOI: https://doi.org/10.1038/sc.2009.62
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