Abstract
Study design:
Experimental Study.
Objectives:
The aim of this study was to investigate the neuroprotective effects of iloprost and piracetam on spinal cord ischemia/reperfusion (I/R) injury in the rabbit.
Settings:
The Experimental Research Center of Selcuk University, Konya, Turkey.
Methods:
A total of 24 rabbits were divided into four groups of six rabbits each, as follows: group 1 (n=6) sham, laparotomy only; group 2 (n=6) I/R; group 3 (n=6) I/R+iloprost; and group 4 (n=6) I/R+piracetam. I/R was established in groups 2, 3 and 4. Subsequently, they were followed up neurologically for 24 h until the rabbits were killed; biochemical and histopathological examinations of samples from the spinal cord were carried out.
Results:
Neurological examination results were significantly better in the iloprost and piracetam groups compared with the I/R group (P<0.05). Neuroprotection was achieved with iloprost and piracetam by suppressing malondialdehyde (P<0.05), increasing glutathione peroxidase activity (P<0.05) and decreasing the xanthine oxidase level. In histopathological assessment, iloprost and piracetam groups were statistically different from the I/R group in terms of the number of apoptotic neurons in gray matter and white matter, as well as in terms of degenerated neurons and glial cells (P<0.05). No statistical difference was determined between the four groups in the number of degenerated glial cells (P>0.05).
Conclusion:
This study has shown that iloprost and piracetam have neuroprotective effects in I/R injury both neurologically and histopathologically because of inhibition of lipid peroxidation.
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References
Kiziltepe U, Turan NN, Han U, Ulus AT, Akar F . Resveratrol, a red wine polyphenol, protects spinal cord from ischemia-reperfusion injury. J Vasc Surg 2004; 40: 138–145.
Erten SF, Kocak A, Ozdemir I, Aydemir S, Colak A, Reeder BS . Protective effect of melatonin on experimental spinal cord ischemia. Spinal Cord 2003; 41: 533–538.
Svensson LG, Rickards E, Coull A, Rogers G, Fimmel CJ, Hinder RA . Relationship of spinal cord blood flow to vascular anatomy during thoracic aortic cross-clamping and shunting. J Thorac Cardiovasc Surg 1986; 91: 71–78.
Tabayashi K, Niibori K, Konno H, Mohri H . Protection from postischemic spinal cord injury by perfusion cooling of the epidural space. Ann Thorac Surg 1993; 56: 494–498.
Zvara DA . Thoracoabdominal aneurysm surgery and the risk of paraplegia: contemporary practice and future directions. J Extra Corpor Technol 2002; 34: 11–17.
Kirsh JR, Helfaer MA, Lange DC, Traystma RJ . Evidence for free radical mechanism of brain injury resulting from ischemia/reperfusion-induced events. J Neurotrauma 1992; 9: 157–163.
Attar A, Tuna H, Sargon MF, Yuceer N, Turker RK, Egemen N . Early protective effects of iloprost after experimental spinal cord injury. Neurol Res 1998; 4: 353–359.
Zivin JA, DeGirolami U . Spinal cord infarction: a highly reproducible stroke model. Stroke 1980; 11: 200–202.
DeGirolami U, Zivin JA . Neuropathology of experimental spinal cord ischemia in the rabbit. J Neuropathol Exp Neurol 1982; 41: 129–149.
Dosluoglu HH, Aktan AO, Yegen C, Okboy N, Yalcm AS, Yahn R et al. The cytoprotective effects of verapamil and iloprost (ZK 36374) on ischemia/reperfusion injury of kidneys. Transpl Int 1993; 3: 138–142.
Takamatsu H, Tsukada H, Watanabe Y, Cui Y, Kataoka Y, Hosoya T et al. Specific ligand for a central type prostacyclin receptor attenuates neuronal damage in a rat model of focal cerebral ischemia. Brain Res 2002; 2: 176–182.
Geber J, Cop J, Cvitanović B, Anić T, Bjegović M . The effect of piracetam on the recurrent inhibition of motor neurones. Neurologija 1990; 39: 163–168.
Keil U, Scherping I, Hauptmann S, Schuessel K, Eckert A, Müller WE . Piracetam improves mitochondrial dysfunction following oxidative stress. Br J Pharmacol 2006; 147: 199–208.
Uchiyama M, Mihara M . Determination of malondialdehyde precursors in tissues by thiobarbituric acid test. Anal Biochem 1978; 86: 279–286.
Kalkan E, Cicek O, Unlu A, Abusoglu S, Kalkan SS, Avunduk MC et al. The effects of prophylactic zinc and melatonin application on experimental spinal cord ischemia–reperfusion injury in rabbits: experimental study. Spinal Cord 2007; 45: 722–730.
Chan PH . Role of oxidants in ischemic brain damage. Stroke 1996; 27: 1124–1129.
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Kalkan, E., Keskin, F., Kaya, B. et al. Effects of iloprost and piracetam in spinal cord ischemia–reperfusion injury in the rabbit. Spinal Cord 49, 81–86 (2011). https://doi.org/10.1038/sc.2010.76
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DOI: https://doi.org/10.1038/sc.2010.76
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