Figure 1: Schematic of MeDIP-chip procedure. | Scientific Data

Figure 1: Schematic of MeDIP-chip procedure.

From: DNA methylation temporal profiling following peripheral versus central nervous system axotomy

Figure 1

Sonicated genomic DNA was immunoprecipitated (IP) with a 5-methylcytosine antibody. IP samples (methylated fragments) and genomic DNA (Input control) samples were amplified supposedly up to 1,000-fold with a Whole Genome Amplification (WGA) Kit (Sigma-Aldrich) and purified. Each corresponding set of IP and Input sample libraries were conjugated with either Cy5 or Cy3, respectively, and co-hybridized on a DNA methylation sensitive tiling microarray (Roche/NimbleGen). Relative IP-to-Input enrichment of methylated DNA fragments was verified by PCR.

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