Figure 2: Global DNA methylation microarray analysis.
From: DNA methylation temporal profiling following peripheral versus central nervous system axotomy
(a) The total hit number of significant methylation events (mean) for all 18,180 annotated genes (by NCBI GeneID) was regarded as a measure for global methylation. The average total hit number for each multiple microarray set is graphed comparing SNA injury (green) or DCA injury (red) to corresponding shams (white), or naive (blue). Triplicate arrays were applied for injury conditions and naive, duplicates for sham. (b) The distribution of gene promoter methylation levels was compared between conditions (bar graph). Most genes were not significantly methylated for each condition, as shown as percentage range in pie charts. The numbers of thus methylated and partially methylated genes were compared (hitting in at least 1/3 arrays for injury or naive, or in at least 1/2 arrays for shams). The average percentage of methylated, partly methylated, or unmethylated genes was similar across all microarrays. The legend for grades of DNA methylation applies for both graphs. Error bars: s.e.; statistics: unpaired two-tailed t-test *P≤0.05, **P≤0.01, ***P≤0.001.
