Figure 5: Quality control of whole-genome-amplified (WGA) samples after MeDIP for DNA methylation microarrays.
From: DNA methylation temporal profiling following peripheral versus central nervous system axotomy
Genomic DNA (Input for MeDIP) was extracted and pooled from each 2 adult mice for each sample of a condition, and sonicated to an average fragment size of approximately 700 bp (100 to 2,000 bp). For each injury condition (SNA/DCA, 3 time points) and naive, a triplicate of pooled samples was used, and duplicate sets for each sham condition. WGA samples displayed a smaller average fragment size of 400 bp (200 to 800 bp for Input, and 150 to 700 bp for IP). As positive control (Pos), non-fragmented commercial human genomic DNA, included in the kit, was whole-genome-amplified as well.
