Table 4 Analytical characterization of the test atmosphere.
From: Comprehensive systems biology analysis of a 7-month cigarette smoke inhalation study in C57BL/6 mice
Parameter | Assay Principle | Determination Schedule |
|---|---|---|
Total particulate matter | Gravimetry after trapping on Cambridge filters | During every exposure block |
Carbon monoxide | Non-dispersive infrared photometry of gas/vapor phase | Continuously |
Nicotine | Capillary gas chromatography after trapping on sulfuric acid-impregnated silica gel | During every exposure block, except during formaldehyde sampling |
Formaldehyde, acetaldehyde, and acrolein | Reversed phase high-performance liquid chromatography of 2,4-dinitrophenylhydrazine (DNPH) derivatives after trapping in DNPH solution | ≥once/week |
Relative humidity | Capacitive | Continuously; sham group only |
Temperature | Thermistor probe Pt100 | Continuously |
(Static) puff volume | Soap bubble flow meter | Before and after daily smoke generation |
Flow rate through exposure chamber | Pressure difference over a Venturi tube | Continuously |
Particle size distribution | PIXE impactor | Once |
