Table 1 Summary of the receptor constructs used for structure determination.

From: Serial femtosecond crystallography datasets from G protein-coupled receptors

Receptor/ligand

N-term tags

N-term truncations

C-term tags

C-term truncations

Mutations

Fusion partner

5-HT2B/ergotamine

HA-Flag

1–35

PP-10 × His

406–481

M144W

BRIL in ICL3 (T249 to V313)

SMO/cyclopamine

HA-Flag-10 × His-TEV

CRD (1–189)

None

556–787

None

BRIL in ICL3 (P434 to K440)

δ-OR/DIPP-NH2

HA-Flag-10 × His-TEV

1–38

None

339–372

None

N-term BRIL

AT1R/ZD7155

HA-Flag-10 × His-TEV

1, 7–16

None

320–359

None

N-term BRIL

  1. 10 × His, 10-mer His tag; BRIL, thermostabilized apocytochrome b562RIL from Escherichia coli (M7W, H102I and R106L); C-term, C terminal; CRD, cysteine-rich domain; Flag, flag tag; HA, hemagglutinin signal sequence; ICL3, intracellular loop 3; N-term, N terminal; PP, prescission protease-recognition site; TEV, TEV protease-recognition site.
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