Table 4 Parameters for hit finding using Cheetah.
From: Serial femtosecond crystallography datasets from G protein-coupled receptors
Receptor/ligand | Minimum number of peaks | Peak criteria | Peak search radii (pixels) | Cheetah peakfinder algorithm | Background subtraction for peak search |
|---|---|---|---|---|---|
5-HT2B/ergotamine | 15 | 2–20 pixels above 180 ADU | 75–3,000 | 3 | Median filter, 3 pixels radius |
SMO/cyclopamine | 15 | 2–20 pixels above 180 ADU | 75–3,000 | 3 | Median filter, 2 pixels radius |
δ-OR/DIPP-NH2 | 15 | 2–40 pixels above 40 ADU with SNR above 4 | 70–700 | 8 | Radial background subtraction |
AT1R/ZD7155 | 15 | 2–40 pixels above 40 ADU with SNR above 4 | 70–700 | 8 | Radial background subtraction |
