Figure 1: Characterization of transcriptional changes in primary human LECs treated with VEGF-C156S by CAGE RNA sequencing.

(a) Schematic overview of the experimental procedure: Primary human dermal LECs from three individual donors were incubated with VEGF-C156S (1.5 μg/ml). RNA was extracted at 16 different time points from 0 min (baseline) to 480 min and subjected to CAGE RNA sequencing. (b) MA plots showing the log₂FC compared to the expression level derived from DESeq2 analysis of differentially expressed CAGE peaks at each time point compared to the baseline. Red dots represent significantly altered CAGE peaks (P<0.05) and horizontal blue lines represent the log₂FC cutoff of 0.6 resp. −0.6. The red numbers in the plots correspond to the number of significantly altered CAGE peaks above the log₂FC cutoff. (c) Summary table of the number of genes corresponding to the DE CAGE peaks. (d) Heat map based on the expression levels (tpm) of all genes corresponding to DE CAGE peaks after hierarchical clustering. Colors code for log₂FC values on a scale from −3 to +3. (e) Venn diagram comparing the upregulated genes between DESeq2 and previously published EdgeR analyses.