Figure 2: Multivariate analysis of the bacterial community from human stool samples.

Non-metric multidimensional scaling (NMDS) based on weighted Unifrac12 was used to display the bacterial community structure in 79 stool samples at same sequencing effort (10.000 reads per sample). Samples from patients who exhibited diarrhea at time of sampling are encased by diamond. Samples from patients that were positively tested on C. difficile by microbiological test are marked by plus, samples of patients where C. difficile was detected in the amplicon dataset are marked by cross. Point size represents the phylogenetic diversity (PD, Faith's Phylogenetic Diversity26) of the microbiome, samples are encircled by PD ranges from 0–10, 10–15, 15–20, and 20–30. Data processing and employed tools are described in detail in the methods section. All alpha diversity metrics obtained by QIIME are listed in Table 3 (available online only).