Table 1 16S rRNA gene sequence processing statistics and biosample accession numbers
Sample name | Biosample accession | Raw reads (2x, paired-end) | After PEAR | % loss* | After QF | % loss * | After taxonomy filter | % loss * |
|---|---|---|---|---|---|---|---|---|
patient_001 | SAMN06011251 | 183,885 | 178,258 | 3.06 | 128,097 | 30.3 | 91,562 | 50.2 |
patient_002 | SAMN06011252 | 283,254 | 272,331 | 3.86 | 208,965 | 26.2 | 189,871 | 33.0 |
patient_003 | SAMN06011253 | 29,313 | 28,372 | 3.21 | 24,528 | 16.3 | 23,857 | 18.6 |
patient_004 | SAMN06011254 | 19,854 | 18,967 | 4.47 | 16,185 | 18.5 | 14,889 | 25.0 |
patient_005 | SAMN06011255 | 77,418 | 73,960 | 4.47 | 60,097 | 22.4 | 56,675 | 26.8 |
patient_006 | SAMN06011256 | 118,110 | 112,829 | 4.47 | 92,535 | 21.7 | 73,601 | 37.7 |
patient_007 | SAMN06011257 | 61,760 | 57,107 | 7.53 | 46,348 | 25.0 | 44,385 | 28.1 |
patient_008 | SAMN06011258 | 188,584 | 183,121 | 2.90 | 160,489 | 14.9 | 151,302 | 19.8 |
patient_009 | SAMN06011259 | 121,488 | 116,373 | 4.21 | 76,062 | 37.4 | 72,353 | 40.4 |
patient_010 | SAMN06011260 | 47,754 | 45,826 | 4.04 | 38,386 | 19.6 | 36,312 | 24.0 |
patient_011 | SAMN06011261 | 1,137,384 | 1,099,480 | 3.33 | 921,087 | 19.0 | 856,364 | 24.7 |
patient_012 | SAMN06011262 | 29,601 | 28,353 | 4.22 | 23,893 | 19.3 | 20,824 | 29.7 |
patient_013 | SAMN06011263 | 603,255 | 575,795 | 4.55 | 470,894 | 21.9 | 419,480 | 30.5 |
patient_014 | SAMN06011264 | 27,890 | 26,906 | 3.53 | 22,664 | 18.7 | 21,306 | 23.6 |
patient_015 | SAMN06011265 | 87,995 | 85,717 | 2.59 | 72,649 | 17.4 | 67,572 | 23.2 |
patient_016 | SAMN06011266 | 81,374 | 77,871 | 4.30 | 65,934 | 19.0 | 56,602 | 30.4 |
patient_017 | SAMN06011267 | 39,762 | 37,969 | 4.51 | 31,481 | 20.8 | 28,623 | 28.0 |
patient_018 | SAMN06011268 | 69,262 | 66,730 | 3.66 | 25,195 | 63.6 | 23,399 | 66.2 |
patient_019 | SAMN06011269 | 83,424 | 80,839 | 3.10 | 67,259 | 19.4 | 63,697 | 23.6 |
patient_020 | SAMN06011270 | 108,817 | 104,597 | 3.88 | 89,081 | 18.1 | 85,928 | 21.0 |
patient_021 | SAMN06011271 | 123,228 | 118,815 | 3.58 | 101,485 | 17.6 | 97,888 | 20.6 |
patient_022 | SAMN06011272 | 27,398 | 26,520 | 3.20 | 21,818 | 20.4 | 19,523 | 28.7 |
patient_023 | SAMN06011273 | 782,019 | 752,476 | 3.78 | 635,823 | 18.7 | 605,208 | 22.6 |
patient_024 | SAMN06011274 | 570,482 | 548,037 | 3.93 | 466,962 | 18.1 | 450,550 | 21.0 |
patient_025 | SAMN06011275 | 29,420 | 28,252 | 3.97 | 23,626 | 19.7 | 22,380 | 23.9 |
patient_026 | SAMN06011276 | 58,090 | 55,621 | 4.25 | 26,390 | 54.6 | 24,952 | 57.0 |
patient_027 | SAMN06011277 | 29,432 | 28,109 | 4.50 | 22,539 | 23.4 | 20,812 | 29.3 |
patient_028 | SAMN06011278 | 181,138 | 173,803 | 4.05 | 140,608 | 22.4 | 126,714 | 30.0 |
patient_029 | SAMN06011279 | 41,796 | 40,543 | 3.00 | 35,367 | 15.4 | 33,444 | 20.0 |
patient_030 | SAMN06011280 | 45,345 | 43,748 | 3.52 | 30,554 | 32.6 | 28,364 | 37.4 |
patient_031 | SAMN06011281 | 48,745 | 47,545 | 2.46 | 13,517 | 72.3 | 12,569 | 74.2 |
patient_032 | SAMN06011282 | 50,655 | 48,039 | 5.16 | 21,797 | 57.0 | 20,248 | 60.0 |
patient_033 | SAMN06011283 | 109,639 | 105,711 | 3.58 | 75,174 | 31.4 | 61,098 | 44.3 |
patient_034 | SAMN06011284 | 19,449 | 18,806 | 3.31 | 16,053 | 17.5 | 14,558 | 25.1 |
patient_035 | SAMN06011285 | 45,502 | 43,883 | 3.56 | 36,311 | 20.2 | 31,231 | 31.4 |
patient_036 | SAMN06011286 | 112,545 | 107,687 | 4.32 | 80,577 | 28.4 | 78,582 | 30.2 |
patient_037 | SAMN06011287 | 26,917 | 25,454 | 5.44 | 20,435 | 24.1 | 19,494 | 27.6 |
patient_038 | SAMN06011288 | 43,597 | 42,515 | 2.48 | 34,868 | 20.0 | 33,540 | 23.1 |
patient_039 | SAMN06011289 | 117,718 | 114,512 | 2.72 | 97,788 | 16.9 | 39,043 | 66.8 |
patient_040 | SAMN06011290 | 73,905 | 69,618 | 5.80 | 54,943 | 25.7 | 52,717 | 28.7 |
patient_041 | SAMN06011291 | 40,730 | 39,301 | 3.51 | 32,688 | 19.7 | 31,371 | 23.0 |
patient_042 | SAMN06011292 | 38,409 | 37,155 | 3.26 | 31,748 | 17.3 | 29,962 | 22.0 |
patient_043 | SAMN06011293 | 13,346 | 13,007 | 2.54 | 11,381 | 14.7 | 10,650 | 20.2 |
patient_044 | SAMN06011294 | 16,592 | 15,995 | 3.60 | 13,586 | 18.1 | 13,207 | 20.4 |
patient_045 | SAMN06011295 | 77,488 | 74,526 | 3.82 | 62,027 | 20.0 | 57,396 | 25.9 |
patient_046 | SAMN06011296 | 66,527 | 64,443 | 3.13 | 51,997 | 21.8 | 50,861 | 23.5 |
patient_047 | SAMN06011297 | 131,863 | 129,034 | 2.15 | 110,888 | 15.9 | 104,209 | 21.0 |
patient_048 | SAMN06011298 | 27,782 | 27,214 | 2.04 | 22,383 | 19.4 | 22,103 | 20.4 |
patient_049 | SAMN06011299 | 18,338 | 17,774 | 3.08 | 14,603 | 20.4 | 13,978 | 23.8 |
patient_050 | SAMN06011300 | 47,408 | 46,252 | 2.44 | 39,412 | 16.9 | 36,086 | 23.9 |
patient_051 | SAMN06011301 | 120,937 | 117,898 | 2.51 | 102,904 | 14.9 | 95,667 | 20.9 |
patient_052 | SAMN06011302 | 59,282 | 57,527 | 2.96 | 49,677 | 16.2 | 47,377 | 20.1 |
patient_053 | SAMN06011303 | 78,974 | 77,534 | 1.82 | 67,320 | 14.8 | 62,539 | 20.8 |
patient_054 | SAMN06011304 | 35,543 | 34,385 | 3.26 | 28,947 | 18.6 | 24,646 | 30.7 |
patient_055 | SAMN06011305 | 95,179 | 92,620 | 2.69 | 75,463 | 20.7 | 70,818 | 25.6 |
patient_056 | SAMN06011306 | 27,168 | 26,425 | 2.73 | 22,551 | 17.0 | 21,338 | 21.5 |
patient_057 | SAMN06011307 | 74,445 | 72,231 | 2.97 | 62,599 | 15.9 | 60,439 | 18.8 |
patient_058 | SAMN06011308 | 329,680 | 317,312 | 3.75 | 268,820 | 18.5 | 257,896 | 21.8 |
patient_059 | SAMN06011309 | 21,033 | 20,266 | 3.65 | 16,779 | 20.2 | 15,706 | 25.3 |
patient_060 | SAMN06011310 | 432,732 | 421,277 | 2.65 | 352,201 | 18.6 | 336,711 | 22.2 |
patient_061 | SAMN06011311 | 24,320 | 23,479 | 3.46 | 18,993 | 21.9 | 18,224 | 25.1 |
patient_062 | SAMN06011312 | 38,558 | 35,981 | 6.68 | 28,868 | 25.1 | 27,283 | 29.2 |
patient_063 | SAMN06011313 | 56,156 | 51,606 | 8.10 | 41,663 | 25.8 | 38,647 | 31.2 |
patient_064 | SAMN06011314 | 46,732 | 44,780 | 4.18 | 34,617 | 25.9 | 33,399 | 28.5 |
patient_065 | SAMN06011315 | 66,685 | 64,446 | 3.36 | 52,337 | 21.5 | 44,968 | 32.6 |
patient_066 | SAMN06011316 | 133,075 | 128,615 | 3.35 | 100,317 | 24.6 | 98,069 | 26.3 |
patient_067 | SAMN06011317 | 172,365 | 166,723 | 3.27 | 142,932 | 17.1 | 137,226 | 20.4 |
patient_068 | SAMN06011318 | 70,140 | 68,535 | 2.29 | 59,169 | 15.6 | 55,802 | 20.4 |
patient_069 | SAMN06011319 | 38,007 | 37,056 | 2.50 | 30,107 | 20.8 | 29,577 | 22.2 |
patient_070 | SAMN06011320 | 13,530 | 13,126 | 2.99 | 11,143 | 17.6 | 10,443 | 22.8 |
patient_071 | SAMN06011321 | 163,830 | 155,275 | 5.22 | 126,207 | 23.0 | 118,940 | 27.4 |
patient_072 | SAMN06011322 | 82,488 | 79,151 | 4.05 | 64,796 | 21.4 | 59,576 | 27.8 |
patient_073 | SAMN06011323 | 1,421,967 | 1,376,514 | 3.20 | 1,126,249 | 20.8 | 676,497 | 52.4 |
patient_074 | SAMN06011324 | 122,784 | 117,609 | 4.21 | 96,116 | 21.7 | 86,400 | 29.6 |
patient_075 | SAMN06011325 | 18,848 | 18,218 | 3.34 | 14,945 | 20.7 | 14,161 | 24.9 |
patient_076 | SAMN06011326 | 34,771 | 33,688 | 3.11 | 27,875 | 19.8 | 26,132 | 24.8 |
patient_077 | SAMN06011327 | 69,092 | 66,520 | 3.72 | 55,769 | 19.3 | 50,121 | 27.5 |
patient_078 | SAMN06011328 | 113,883 | 111,135 | 2.41 | 98,305 | 13.7 | 92,503 | 18.8 |
patient_079 | SAMN06011329 | 44,406 | 42,365 | 4.60 | 34,012 | 23.4 | 31,744 | 28.5 |