Figure 1: The distribution of read lengths in the Poly(A)-selected samples.

The average distribution of read lengths which align to the human (hg19) genome is shown in a (n=7), and for the HCMV genome (FJ616285.1) in b (n=7). The same can be seen broken down to the three sample complexes in c and d (for the hg19 and the FJ616285.1 genomes respectively). The sample complex PolyA1 was used for three SMRTcells, PolyA2 and PolyA3 were used for two SMRTcells each. Error bars represent s.e.