Figure 1: Data generation workflow. | Scientific Data

Figure 1: Data generation workflow.

From: Leukocyte Tracking Database, a collection of immune cell tracks from intravital 2-photon microscopy videos

Figure 1

(a) in vivo imaging acquisition (left) Surgically exposed tissues from an anaesthetized and immobilized mouse are subjected to MP-IVM. (right) 4D data composed by z-stack of parallel image planes are acquired at different time points for multiple channels. (b) Cell detection and tracking (left) Cells are detected and the centroid position annotated the image series (red dots). Subsequently, (right) centroids are associated over time producing the cell tracks. For each phase of the imaging pipeline are reported specific problematics that affect cell tracking. c. Database. Both 4D imaging data and cell tracks are included in LTDB.

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