Figure 2: Tracking challenges.
(a) Example case studies (i-viii) Representative snapshots of selected MP-IVM micrographs from problematic cases indicated in the upper part of the picture. (i) Surface reconstruction (SR) (green) of a T cell with uropodia. (ii) SR (red) of a T cell migrating with pseudopodia on a high background. (iii) SR (yellow, red) of two neutrophils forming a brief contact. (iv) Centers of mass (red dots) of a T cell forming a brief contact with a non motile object. (v) Estimated center of mass (red dot) of a T cell close to a boundary of the field of view. (vi) SR (blue) of a rapidly migrating Neutrophil. Arrows indicate cell displacement. (vii) SR (colored objects) of Neutrophils forming multiple contacts. (viii) Tracks (colored lines) of two B cells. (b) Effect of tracking errors Tracking errors limit research reproducibility, significantly () affecting the typical readouts from MP-IVM experiments. The figures (top-left and top-right) and the graphs (bottom) compare the manual tracks presented in LTDB017_a vs. the tracks generated automatically by Imaris. Automatic tracks were interrupted when the software could not detect or link cells, yielding to the creation of an increased number of shorter tracklets.
