Table 3 Channel specification Description of which cell population is expected to be visible in each channel of the provided videos.

From: Leukocyte Tracking Database, a collection of immune cell tracks from intravital 2-photon microscopy videos

VideoID

CH0

CH1

CH2

CH3

CS001

BG(FR)

Tc(CMTMR)

a:Tc(HIV-GFP)

Coll

CS002

BG(FR)

Tc(CMTMR)

a:Tc(HIV-GFP)

Coll

CS003

BG(FR)

Tc(CMTMR)

a:Tc(HIV-GFP)

Coll

CS004

a:Ne(UBC-CFP), Dc(CD11c YFP)

Vaccine

a:Ne(UBC-CFP), Coll

 

CS005

a:Ne(UBC-GFP)

   

CS006

BG(FR)

Tc(CMTMR)

a:Tc(HIV-GFP)

Coll

CS007

a:Ne(UBC-GFP)

   

CS008

a:Ne(UBC-GFP)

   

CS009

a:Ne(UBC-GFP)

   

CS010

a:Ne(UBC-CFP), Dc(CD11c YFP)

Vaccine

a:Ne(UBC-CFP), Coll

 

CS011

a:Ne(UBC-GFP), BG

BG

Coll, BG

 

CS012

a:Tc(CFSE)

   

CS013

a:Ne(UBC-CFP), Dc(CD11c YFP)

Vaccine

a:Ne(UBC-CFP), Coll

 

CS014

a:Ne(UBC-GFP)

Vaccine

a:Ne(UBC-CFP), Coll

 

CS015

a:Ne(UBC-CFP), Dc(CD11c YFP), AF

Ne(CMTMR), AF

a:Ne(UBC-CFP), Coll, AF

 

CS016

a:Ne(UBC-CFP), Dc(CD11c YFP), AF

Ne(CMTMR), AF

a:Ne(UBC-CFP), Coll, AF

 

CS017

a:Ne(UBC-CFP), Dc(CD11c YFP), AF

Ne(CMTMR), AF

a:Ne(UBC-CFP), Coll, AF

 

CS018

a:NK(NCR1 GFP), Mp(CD169 Pe)

Mp(Cd169 Pe)

Vaccine

Coll

LTDB001

a:Ne(UBC-CFP), Dc(CD11c YFP)

Vaccine

a:Ne(UBC-CFP), Coll

 

LTDB002

a:Ne(UBC-CFP), Dc(CD11c YFP)

Vaccine

a:Ne(UBC-CFP), Coll

 

LTDB003

a:Ne(UBC-CFP), Dc(CD11c YFP)

Vaccine

a:Ne(UBC-CFP), Coll

 

LTDB004

Ne(UBC-GFP), a:Ne(UBC-CFP), AF

b:Ne(CMTMR), AF

a:Ne(UBC-CFP), Coll, AF

 

LTDB005

Ne(UBC-GFP), a:Ne(UBC-CFP), AF

b:Ne(CMTMR), AF

a:Ne(UBC-CFP), Coll, AF

 

LTDB006

a:Ne(UBC-CFP), Dc(CD11c YFP)

Vaccine, BG

a:Ne(UBC-CFP), Coll

 

LTDB007

b:Ne(UBC-GFP), a:Ne(UBC-CFP), AF

Ne(CMTMR), AF

a:Ne(UBC-CFP), Coll, AF

 

LTDB008

a:Ne(UBC-CFP), Dc(CD11c YFP)

Vaccine

a:Ne(UBC-CFP), Coll

 

LTDB009

a:Ne(UBC-CFP), Dc(CD11c YFP)

Vaccine

a:Ne(UBC-CFP), Coll

 

LTDB010

Ne(UBC-GFP), BG

a:Bc(CTV)

  

LTDB011

a:Ne(UBC-GFP), BG

Bc(CD19 RFP)

  

LTDB012

BG(FR)

a:Tc(CMTMR)

b:Tc(HIV-GFP)

Coll

LTDB013

BG(FR)

Tc(CMTMR)

a:Tc(HIV-GFP)

Coll

LTDB014

b:Bc(Bodipy 650)

Tc(H2B-RFP)

Tc(NFAT-GFP)

a:Bc(Ag+ Hoechst33342)

LTDB015

Tc(CMAC)

a:Tc(CFSE)

Tc(CMTMR)

HEV(Meca-633)

LTDB016

a:Ne(UBC-CFP), Dc(CD11c YFP)

Ne(CMTMR), AF

a:Ne(UBC-CFP), Coll, AF

 

LTDB017

a:Ne(UBC-CFP), Dc(CD11c YFP)

Ne(CMTMR), AF

a:Ne(UBC-CFP), Coll, AF

 

LTDB018

Dc(CD11c YFP)

a:NK(CMTMR)

  

LTDB019

a:NK(CTV), Dc(CD11c YFP)

Vaccine

a:NK(CTV)

 

LTDB020

BG

a:NK(CMTMR)

BG

Coll

  1. Bold indicates the cells that have been tracked. Brackets reports the type of staining used. The prefix a: or b: is used to indicate which is the corresponding tracking file (e.g. for video 04, b:N.(CMTMR) means that the tracks relative to the neutrophils labelled with CMTMR are in 04b.csv). Legend: Bc = B cells, Tc = T cells, Ne = neutrophils, Dc = dendritic cells, NKs= natural killer cells, Mp = macrophages, AF = auto-fluorescence, BG = background, Coll = collagen.
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