Table 6 Mouse strains Specification of mouse strains used as host and as source of cells for each video.
VideoID | Host | Host provider | Host reference | Cells | Cell source | Cell source reference | Comment |
|---|---|---|---|---|---|---|---|
CS001 | BLT NOD.scid humanized mice | MGH Humanized mouse core | CD4+ T cells | BLT NOD.scid humanized mice | central memory-like CD4+ T cells were isolated and expanded from syngeneic BLT humanized mice, infected with HIV-GFP or labeled with Celltracker Orange (CMTMR) and injected to the host | ||
CS002 | BLT NOD.scid humanized mice | MGH Humanized mouse core | CD4+ T cells | BLT NOD.scid humanized mice | central memory-like CD4+ T cells were isolated and expanded from syngeneic BLT humanized mice, infected with HIV-GFP or labeled with Celltracker Orange (CMTMR) and injected to the host | ||
CS003 | BLT NOD.scid humanized mice | MGH Humanized mouse core | CD4+ T cells | BLT NOD.scid humanized mice | central memory-like CD4+ T cells were isolated and expanded from syngeneic BLT humanized mice, infected with HIV-GFP or labeled with Celltracker Orange (CMTMR) and injected to the host | ||
CS004 | CD11c-EYFP | Jackson | Neutrophils | UBC-CFP mice | Neutrophils expressing CFP were isolated from CK6/ECFP mice and injeted to the host animal | ||
CS005 | C57BL/6Jrj | Janvier laboratories | Neutrophils | UBC-GFP mice | Neutrophils expressing GFP were isolated from UBC-GFP mice and injected to the host animal | ||
CS006 | BLT NOD.scid humanized mice | MGH Humanized mouse core | CD4+ T cells | BLT NOD.scid humanized mice | central memory-like CD4+ T cells were isolated and expanded from syngeneic BLT humanized mice, infected with HIV-GFP or labeled with Celltracker Orange (CMTMR) and injected to the host | ||
CS007 | C57BL/6Jrj | Janvier laboratories | Neutrophils | UBC-GFP mice | Neutrophils expressing GFP were isolated from UBC-GFP mice and injected to the host animal | ||
CS008 | LysMcre GFP | Jackson | Neutrophils | host | |||
CS009 | CD19-RFP | Thelen Lab, IRB Bellinzona | Neutrophils | LysMcre GFP mice | Gonzalez lab, IRB Bellinzona | Neutrophils expressing GFP were isolated from UBC-GFP mice and injected to the host animal | |
CS010 | CD11c-EYFP | Jackson | Neutrophils | UBC-CFP mice | Neutrophils expressing CFP were isolated from CK6/ECFP mice and injeted to the host animal | ||
CS011 | LysMcre GFP | Jackson | Neutrophils | host | |||
CS012 | C57BL/6J | Charles River | https://www.criver.com/products-services/find-model/jax-c57bl6j-mice?region=3616 | T Cells | Ndr DKO mice | TKI, Bern | Ndr DKO mice were generated by crossing Ndr1−/−Ndr2f/f mice with mice expressing cre recombinase driven by the lck proximal promoter [Jackson Laboratory, B6.Cg-Tg (Lck-cre; 548Jxm/J stock #003802)]. All of the mice used for experiments were backcrossed to C57BL/6J mice for at least six generations. The C57BL/6J mice used for adoptive trans- fer experiments were purchased from Charles River. |
CS013 | CD11c-EYFP | Jackson | Neutrophils | UBC-CFP mice | Neutrophils expressing CFP were isolated from CK6/ECFP mice and injeted to the host animal | ||
CS014 | C57BL/6Jrj | Janvier laboratories | Neutrophils | UBC-CFP mice, UBG-GFP mice | https://www.jax.org/strain/004218, https://www.jax.org/strain/004353 | Neutrophils expressing CFP were isolated from CK6/ECFP mice and neutrophils expressing GFP were isolated from UBC-GFP mice. Both were injeted to the host animal. Additionally, the host animal was injected with CD169 PE | |
CS015 | CD11c-EYFP | Jackson | Neutrophils | UBC-CFP mice, P2rx7-/- mice | https://www.jax.org/strain/004218, https://www.jax.org/strain/005576 | Cells were isolated from a UBC-CFP animal and from a P2rx7-/- animal labeled with CMTMR, then injected to the host animal | |
CS016 | CD11c-EYFP | Jackson | Neutrophils | UBC-CFP mice, P2rx7-/- mice | https://www.jax.org/strain/004218, https://www.jax.org/strain/005576 | Cells were isolated from a UBC-CFP animal and from a P2rx7-/- animal labeled with CMTMR, then injected to the host animal | |
CS017 | CD11c-EYFP | Jackson | Neutrophils | UBC-CFP mice, P2rx7-/- mice | https://www.jax.org/strain/004218, https://www.jax.org/strain/005576 | Cells were isolated from a UBC-CFP animal and from a P2rx7-/- animal labeled with CMTMR, then injected to the host animal | |
CS018 | Ncr1 GFP | Jackson | Natural killer cells | host | |||
LTDB001 | CD11c-EYFP | Jackson | Neutrophils | UBC-CFP mice | Neutrophils expressing CFP were isolated from CK6/ECFP mice and transfected to the host animal | ||
LTDB002 | CD11c-EYFP | Jackson | Neutrophils | UBC-CFP mice | Neutrophils expressing CFP were isolated from CK6/ECFP mice and transfected to the host animal | ||
LTDB003 | CD11c-EYFP | Jackson | Neutrophils | UBC-CFP mice | Neutrophils expressing CFP were isolated from CK6/ECFP mice and transfected to the host animal | ||
LTDB004 | C57BL/6Jrj | Janvier laboratories | Neutrophils | UBC-CFP mice, UBC-GFP mice, C57BL/6Jrj mice | https://www.jax.org/strain/004218, https://www.jax.org/strain/004353, https://www.janvier-labs.com/rodent-research-models-services/research-models/per-species/inbred-mice/product/c57bl6jrj.html | Neutrophils expressing CFP were isolated from CK6/ECFP mice, Neutrophils expressing GFP were isolated from UBC-GFP mice. Neuttrophils were isolated from B6 animals and labeled with CMTMR. The three different cells were injeted to the host animal. | |
LTDB005 | C57BL/6Jrj | Janvier laboratories | Neutrophils | UBC-CFP mice, UBC-GFP mice, C57BL/6Jrj mice | https://www.jax.org/strain/004218, https://www.jax.org/strain/004353, https://www.janvier-labs.com/rodent-research-models-services/research-models/per-species/inbred-mice/product/c57bl6jrj.html | Neutrophils expressing CFP were isolated from CK6/ECFP mice, Neutrophils expressing GFP were isolated from UBC-GFP mice. Neuttrophils were isolated from B6 animals and labeled with CMTMR. The three different cells were injeted to the host animal. | |
LTDB006 | C57BL/6Jrj | Janvier laboratories | Neutrophils | UBC-CFP mice, UBC-GFP mice, C57BL/6Jrj mice | https://www.jax.org/strain/004218, https://www.jax.org/strain/004353, https://www.janvier-labs.com/rodent-research-models-services/research-models/per-species/inbred-mice/product/c57bl6jrj.html | Neutrophils expressing CFP were isolated from CK6/ECFP mice, Neutrophils expressing GFP were isolated from UBC-GFP mice. Neuttrophils were isolated from B6 animals and labeled with CMTMR. The three different cells were injeted to the host animal. | |
LTDB007 | C57BL/6Jrj | Janvier laboratories | Neutrophils | UBC-CFP mice, UBG-GFP mice | https://www.jax.org/strain/004218, https://www.jax.org/strain/004353 | Neutrophils expressing CFP were isolated from CK6/ECFP mice and neutrophils expressing GFP were isolated from UBC-GFP mice. Both were injeted to the host animal. Additionally, the host animal was injected with CD169 PE | |
LTDB008 | CD11c-EYFP | Jackson | Neutrophils | UBC-CFP mice | Neutrophils expressing CFP were isolated from CK6/ECFP mice and transfected to the host animal | ||
LTDB009 | CD11c-EYFP | Jackson | Neutrophils | UBC-CFP mice | Neutrophils expressing CFP were isolated from CK6/ECFP mice and transfected to the host animal | ||
LTDB010 | LysMcre GFP | Jackson | B Cells | LysMcre-GFP mice | Gonzalez lab, IRB Bellinzona | B Cells were isolated from non-GFP littermates (coming from the LysM-Cre-GFP crossings) and labelled with Celltracker violet | |
LTDB011 | CD19-RFP | Thelen Lab, IRB Bellinzona | Neutrophils | LysMcre GFP mice | Gonzalez lab, IRB Bellinzona | Neutrophils expressing GFP were isolated from LysMcre-GFP mice and injected to the host animal | |
LTDB012 | BLT NOD.scid humanized mice | MGH Humanized mouse core | CD4+ T cells | BLT NOD.scid humanized mice | MGH Humanized mouse core | central memory-like CD4+ T cells were isolated and expanded from syngeneic BLT humanized mice, labelled with Celltracker green and injected to the host | |
LTDB013 | BLT NOD.scid humanized mice | MGH Humanized mouse core | CD4+ T cells | BLT NOD.scid humanized mice | MGH Humanized mouse core | central memory-like CD4+ T cells were isolated and expanded from syngeneic BLT humanized mice, infected with HIV-GFP or labeled with Celltracker Orange (CMTMR) and injected to the host | |
LTDB014 | BALB/c | Jackson | CD8+ T cells, Ag+ B cells, B Cells | CL4 mice, BALB/c mice | MGH, Jackson | Balb/c (Jackson) to which HA-specific CD8 (purified from CL4 mice, bred in house at Massachusetts General Hospital) transduced with NFAT-GFP and H2B-RFP were transferred 2 days before imaging. Upon imaging, two populations of differentially labeled B cells (purified from Balb/c mice, Jackson) were also transferred. | |
LTDB015 | C57BL/6J | Charles River | https://www.criver.com/products-services/find-model/jax-c57bl6j-mice?region=3616 | T Cells | Ndr DKO mice | TKI Bern | Ndr DKOmicewere generated by crossing Ndr1−/−Ndr2f/f mice with mice expressing cre recombinase driven by the lck proximal promoter [Jackson Laboratory, B6.Cg-Tg (Lck-cre; 548Jxm/J stock #003802)]. All of the mice used for experiments were backcrossed to C57BL/6J mice for at least six generations. The C57BL/6J mice used for adoptive trans- fer experiments were purchased from Charles River. |
LTDB016 | CD11c-EYFP | Jackson | Neutrophils | UBC-CFP mice, P2rx7-/- mice | https://www.jax.org/strain/004218, https://www.jax.org/strain/005576 | Cells were isolated from a UBC-CFP animal and from a P2rx7-/- animal labeled with CMTMR, then injected to the host animal | |
LTDB017 | CD11c-EYFP | Jackson | Neutrophils | UBC-CFP mice, P2rx7-/- mice | https://www.jax.org/strain/004218, https://www.jax.org/strain/005576 | Cells were isolated from a UBC-CFP animal and from a P2rx7-/- animal labeled with CMTMR, then injected to the host animal | |
LTDB018 | CD11c-EYFP | Jackson | Natural killer cells | C57BL/6Jrj | Janvier laboratories | Natural killer cells were isolated from a B6 animal, labelled with CMTMR and injected to the host animal | |
LTDB019 | CD11c-EYFP | Jackson | Natural killer cells | C57BL/6Jrj | Janvier laboratories | Natural killer cells were isolated from a B6 animal, labelled with Celltracker violet and injected to the host animal | |
LTDB020 | IFNg eYFP | Jackson | Natural killer cells | C57BL/6Jrj | Janvier laboratories | Natural killer cells were isolated from a B6 animal, labelled with CMTMR and injected to the host animal |
