Figure 1: Overview of MANTA2.

a) Intersection of the ReMap ChIP-seq regions with JASPAR TFBS predictions to produce a set of TFBSs with both experimental and computational evidence of TF binding. A mock example of JUN is given for a region on chromosome one. b) A matrix representing the difference in PWM score for all possible SNVs compared to the reference sequence at that TFBS, including negative impact (−), positive impact (+), and no change (0) of score. Black boxes indicate that nucleotides of the reference TFBS sequence are not stored in the database. The sequence logo for JUN is provided below the matrix where the information content is proportional to the size of the nucleotide letters. c) Mock distribution of TFBS SNV impact scores when considering all possible SNVs in the TFBS. The distribution is annotated with examples of decreased TF binding capacity (red), no change in TF binding capacity (yellow), and increased TF binding capacity (green).