Figure 4: Line graphs of hybridisation controls.
From: Microarray data of transcriptome shifts in blood cell subsets during S1P receptor modulator therapy
Premixed spikes were added to the samples at two time points: before the enzymatic steps (labelling controls) and during the preparation of the hybridisation cocktail (hybridisation controls). They served to control the proper enzymatic conversion of transcripts and the correct conditions during microarray hybridisation, washing, staining and scanning. The labelling controls are sequences derived from Bacillus subtilis genes (Lys: AFFX-r2-Bs-lys (1:100,000), Phe: AFFX-r2-Bs-phe (1:50,000), Thr: AFFX-r2-Bs-thr (1:25,000), Dap: AFFX-r2-Bs-dap (1:6,667)). The default hybridisation controls are composed of BioB, BioC, BioD and Cre in staggered concentrations (AFFX-r2-Ec-BioB, AFFX-r2-Ec-BioC, AFFX-r2-Ec-BioD, AFFX-r2-P1-Cre). The two graphs are depicting the respective log2 values after applying SST-RMA. Consistency across all 150 samples is demonstrated by increasing signal values (Lys<Phe<Thr<Dap and BioB<BioC<BioD<Cre). The colour code distinguishes the 5 different cell populations studied.
