Figure 4: Quality control of the single cell sequencing library preparation.
From: Single-cell RNA sequencing of mouse brain and lung vascular and vessel-associated cell types
(a) Representative cDNA graphs from a validation plate, analyzed with a High Sensitivity D5000 ScreenTape on a TapeStation 4200. The size distribution of the cDNA was established by running a ladder (top left). A1 and A2 represent positive controls (20 cells), while P1 and P2 are empty negative controls. The other graphs display cDNA size distribution of randomly picked single cells of a validation plate after amplification. Graph E1 has colored boxes to assist in clarifying peak significance: Red boxes indicate upper and lower markers for size selection, the green box shows the cDNA size distribution and the blue box highlights the primer dimers. (b) Quality control of the pooled sequencing library after tagmentation with homemade Tn5 and indexing with the Nextera Indexing XT kit. The sequencing pools were analyzed on a BioAnalyzer with a High Sensitivity DNA Chip. Colored boxes describe peak significance as described above.
