Figure 1: Cardiomyogenic differentiation of hESCs.
From: Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs
(a) Schematic representation of the steps followed for RNA-seq data generation. (b) Schematic representation of the cardiomyogenic differentiation protocol, indicating days of differentiation and timing of specific induction. (c) Representative images of EBs during differentiation showing NKX2-5/eGFP expression on D15. Phase contrast (PC) and eGFP fluorescence (left image), eGFP fluorescence (right image). 250 μm scale. (d) Representative images of differentiated cardiomyocytes stained for cTnI on D20. Isotype control (left image), cTnI staining (right image). White rectangle as 50 μm scale.
