Table 4 Illumina RNA sequencing metrics, before and after preprocessing.

From: The draft genome sequence of cork oak

Plant Tissue

Number of libraries

Original number of reads

Reads kept after pre-processing

Percentage of reads kept

Pollen

1

197,725,257

192,418,402

97.3

Leaf

2

299,960,018

280,640,162

93.6

Xylem

2

361,255,569

338,218,694

93.6

Inner bark

2

311,378,053

291,162,581

93.5

Phellem

2

360,128,704

335,696,318

93.2

  1. The reads were trimmed, when required, to a minimum length of 80 nucleotides, using Sickle13 and a minimum quality of 20.