Table 1 Primer sequences used in this study.
Gene ID |  |  | Primer sequnces (5' → 3') | Product size | Application |
|---|---|---|---|---|---|
igf3 | igf3 iF | F | TAATACGACTCACTATAGGGTGTACTGCGTCCTGATCCTG | 482 bp | RNA interference |
igf3 iR | R | TAATACGACTCACTATAGGGGAAGGTTGCTGCTGTGTTGA | |||
igf3 | F | AGCAGCGATACCAGAAGCAT | 91 bp | qRT-PCR | |
igf3 | R | GAGTTCCACCGGTAAAGCGT | |||
lncRNA | MSTRG.42108 | F | GCTCCAGCTTGATCCTCATC | 81 bp | |
R | GAGACGAGCTGAAGCCTTTC | ||||
MSTRG.13209 | F | CATTCTCCATGTAGGGCTCC | 143 bp | ||
R | ACTGGAGGAGCTGAGAACCA | ||||
MSTRG.10998 | F | ACCTTTAAACAAACGGCACG | 150 bp | ||
R | CCAACTGGGATCCTGAAGAA | ||||
MSTRG.43997 | F | GACACCTTGGGAGGAACTGA | 198 bp | ||
R | CTTTGCTTTTCACCTCAGGG | ||||
MSTRG.7759 | F | GCAAGCCATCTCTCGACTCT | 95 bp | ||
R | AGCAGCATCTCCTTCAGCTC | ||||
MSTRG.43046 | F | AGGAGTGAAGTCCTGAGGCA | 148 bp | ||
R | GATCCCCGTTCACTGTCTGT | ||||
MSTRG.19541 | F | AGCCGACAATAGAGCGGACT | 166 bp | ||
R | CAGAATCGCTCCCGTTTGAAG | ||||
MSTRG.36412 | F | AAGGCCACGAGATGAAGAGA | 127 bp | ||
R | GCCCAGCAATAAAAGGAACA | ||||
MSTRG.29920 | F | AAGGGGGAGGTCAACTTGGT | 103 bp | ||
R | ACAGAGCCTGGAGAGCCATT | ||||
MSTRG.16585 | F | GTCTTAGGAGGACGGGACGA | 125 bp | ||
R | CCGTGTCACGTGAGTTCTCC | ||||
MSTRG.48206 | F | GCCCCTTGTGTTTTTCTCAA | 169 bp | ||
R | TCATTTTGGAGGGTGGAAAT | ||||
MSTRG.22976 | F | GCTCCAGTGGACCGTAAAGA | 92 bp | ||
R | CAGATGGAGGTCCTGGAGAA | ||||
MSTRG.2504 | F | ACAAGCTTTGGTCTGGACATTT | 99 bp | ||
R | GCTCTTCTGTTCATTTCGACCT | ||||
MSTRG.40241 | F | AACGCCATCTTCATTCGTGT | 144 bp | ||
R | AAAACTCCTGCTGAAGCGAC | ||||
MSTRG.36182 | F | GCGAAGAAGAACAGTTTGCC | 155 bp | ||
R | ACAATCCTTCCCGAGTCAGA | ||||
MSTRG.23436 | F | TTTTGTAACCCCCAGCTTTG | 128 bp | ||
R | TTTACAGACCCCCTTTCCCT | ||||
MSTRG.27815 | F | TCAGGGGAAGTGTTGCTTCT | 95 bp | ||
R | GCTGAGCCGTTTCAGAAAGT | ||||
MSTRG.7538 | F | AATGGCTGGGTGTGTGTGTA | 181 bp | ||
R | TGTCGAATATCTGCGGGAAT | ||||
MSTRG.51187 | F | AGACGGGGGCGAGAATACAA | 120 bp | ||
R | CACTAATCCTGGCCCTGGGA | ||||
MSTRG.18820 | F | GGAGACAGCAGGGAACACAT | 178 bp | ||
R | GTCCGTGTTTTGCTCCTCAT | ||||
mRNA | CAFS_CommonCarp_T_00220460 | F | CCCGTGTGTTTCATTGCTGA | 200 bp | |
R | GTGCAGGCAGGATCATTGAG | ||||
CAFS_CommonCarp_T_00106217 | F | TGCTATCGAGTGGCAAACTG | 221 bp | ||
R | TTAAGGGGAGGAGAGCCATT | ||||
CAFS_CommonCarp_T_00276083 | F | CTGGACCAAGAGTGGTGGTT | 137 bp | ||
R | CACCAAACTCCCTCCTGAAA | ||||
CAFS_CommonCarp_T_00055524 | F | CTGGGAGGTGGACAAGTCAT | 228 bp | ||
R | TTCTGGAGGTGGCGTTAGTT | ||||
CAFS_CommonCarp_T_00253405 | F | ACACCCCACCTCACAGAGAC | 143 bp | ||
R | GGTCACCAACCTGTTCTCGT | ||||
CAFS_CommonCarp_T_00128677 | F | AAACCTACACGTGTGGAGCC | 194 bp | ||
R | CGGTACGTGGTTCCTCTGTT | ||||
CAFS_CommonCarp_T_00047108 | F | ATTCGGGCGTAGCACAATAC | 81 bp | ||
R | ATGTCACTTTCTGGGATCGG | ||||
CAFS_CommonCarp_T_00071301 | F | CTCAGGGGGAAAAACAAACA | 123 bp | ||
R | AAACTCCGTGCAATACGACC | ||||
CAFS_CommonCarp_T_00083670 | F | AGACGAAGGAGGGAGGAGAG | 179 bp | ||
R | CCGAGGATGATCTCACCAGT | ||||
CAFS_CommonCarp_T_00206701 | F | CTATGACAAAGCCTTCCCCA | 131 bp | ||
R | GCTGACGTTCTTTTTCGAGG | ||||
CAFS_CommonCarp_T_00037501 | F | TGCATCAGAGTTTCACTGCC | 117 bp | ||
R | GACGGACTGACAGCAGAACA | ||||
CAFS_CommonCarp_T_00190084 | F | CACCGGTCAGAAACCGTACT | 165 bp | ||
R | ATCCGCTTGTGGATTTTCAG | ||||
CAFS_CommonCarp_T_00208822 | F | GCAAAAGAGTGTGTGCAGGA | 166 bp | ||
R | AGAGTTTAAGCGGCTCCACA | ||||
CAFS_CommonCarp_T_00088409 | F | CAGCATTCTGAAAGGAAGGC | 167 bp | ||
R | ATCCTGTTTTCGCCAACATC | ||||
CAFS_CommonCarp_T_00213038 | F | GAGACCCAAAATCAAGCCAA | 125 bp | ||
R | TACGGTGTGGTGTTGAGCAT | ||||
CAFS_CommonCarp_T_00239240 | F | CTGAAGCAGGAGTACCAGCC | 87 bp | ||
R | GTCAGCACCTTTACCCAGGA | ||||
CAFS_CommonCarp_T_00112860 | F | GCCAAGCACAACACTGCTTA | 152 bp | ||
R | GGACAACTTGGGGAGTTTGA | ||||
CAFS_CommonCarp_T_00038343 | F | TCAGAACGTGGTGTCTGAGC | 181 bp | ||
R | TTGTACGAGGAGCTGCAATG | ||||
CAFS_CommonCarp_T_00052776 | F | CGAAAACCCAGCAGTTCTGT | 149 bp | ||
R | TGCTGCCCTCTCGATAACTT | ||||
CAFS_CommonCarp_T_00128308 | F | GTGATCAGCGCTGTGATGTT | 122 bp | ||
R | CCTGTCACGAGAATGCAGAA | ||||
β-actin | β-actin | F | GCTATGTGGCTCTTGACTTCGA | 89 bp | |
R | CCGTCAGGCAGCTCATAGCT |
