Figure 5: Validation of the cell-type specificity in the Pitx3-tTA line. | Scientific Data

Figure 5: Validation of the cell-type specificity in the Pitx3-tTA line.

From: Brain-wide distribution of reporter expression in five transgenic tetracycline-transactivator mouse lines

Figure 5

(a) X-gal stained coronal section from a Pitx3-tTA/tetO-lacZ-nls-GFP mouse brain, with atlas derived annotations shown in the left hemisphere. (b) Enlarged detail from (a) showing part of the midbrain containing the substantia nigra. (c–e) Confocal microscopic images with increasing magnification of a green fluorescent protein (GFP), tyrosine hydroxylase (TH) and DAPI stained section adjacent to the section in (a,b). TH immunohistochemistry is used to identify dopaminergic cells in the brain, and TH positive cells appear red in (c–e). Cells co-labeled with GFP and TH (arrows) in the SNc are shown in (d) and a single co-labeled cell in (e). Note that GFP, and thus also lacZ, is expressed only in a subset of TH positive cells. SNc, substantia nigra compact part; SNr, substantia nigra reticular part; VTA, ventral tegmental area. Scale bars, (a) 1 mm (b,c) 100 μm (d) 20 μm (e), and 2 μm.

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