Figure 7: Validation of cell-type specificity in the Pcp2-tTA line.

(a) X-gal stained horizontal section from a Pcp2-tTA/tetO-lacZ-nls-GFP mouse brain. The left hemisphere has annotations corresponding to a virtual atlas map from the Allen Mouse CCF v.2. The cartoon drawing of a sagittal section shows the approximate cutting plane as a red line. (b) Enlarged detail from the section in (a) showing part of the cerebellum. (c) Further magnified detail from the section in (a,b) showing labeling between the cerebellar granular layer and the molecular layer. (d) Confocal microscopic image of a green fluorescent protein (GFP), calbindin, and DAPI stained section adjacent to the section in (a–c). Calbindin is a marker for Purkinje cells, and with high magnification one can clearly identify cells co-labeled for GFP and calbindin (e). Since expression of GFP and lacZ are both driven by the Pcp2 promoter, this verifies that the X-gal labeled cells in (a–c) are also Purkinje cells. AUD, primary auditory area; Crus 1, cerebellar crus 1, GL, granular cell layer; ML, molecular cell layer; MO, primary motor area; PAG, periaqueductal gray; PC, Purkinje cell; PCL, Purkinje cell layer; SS, primary somatosensory area; Sim, simple lobule. Scale bars, (a) 1 mm (b,d), and 200 μm (c,e) 20 μm.