Figure 8: Examples of use.

(a–d) X-gal labeled coronal sections through the midbrain from Prnp-tTA/tetO-lacZ (a,c) and Pitx3-tTA/tetO-lacZ-nls-GFP (b,d) mice. The left hemispheres are presented with semi-transparent overlays of custom atlas maps as available in the online viewer. (c,d) Higher-magnification details from the substantia nigra showing notably different labeling patterns between the two lines. In this region the Prnp-tTA line mainly has labeled cells within the reticular part (c), while the Pitx3-tTA line displays labeling highly restricted to the compact part and the ventral tegmental area. (d). Note that for the Prnp-tTA line, labeling also occurs in several other regions such as the hippocampal formation and the cerebral cortex. (e) Shows a screenshot of in situ hybridization data representing gene distribution of the Camk2a gene in the hippocampal area of a normal mouse from the Allen Institute repository (www-brain-map.org). (f) Shows a screenshot of a corresponding X-gal labeled section from a Camk2a-tTA/tetO-lacZ mouse, available from the rodent brain workbench. Similar viewers facilitate comparison of native gene distribution patterns and tTA dependent transgene expression patterns. Ctx, cerebral cortex; HF, hippocampal formation; SC, superior colliculus; SN, substantia nigra; SNc, substantia nigra, compact part; SNr, substantia nigra, reticular part; Th, thalamus; VTA, ventral tegmental area. Scale bars, 1 mm (a,b), 100 μm (c,d).